1) Introduction to GLP, GCP and GMP
Course No: 2542, 1,5 hp. Course leader: Evren Alici
The aim for the course is to teach the students advances in Good Laboratory Practice (GLP), Good manufacturing practice (GMP) and basics in good clinical practice (GCP). Ethical aspects regarding animal and clinical research will also be briefly covered. Finally, there will be a session regarding the Swedish and European biobanking rules and regulations. At the end of the course, the participant will have a detailed knowledge on: Rules, regulations and ethics in laboratory environments, biobanking ethics and routines for first-in-man clinical trials, practical and theoretical basis for GMP, ethical aspects of translational research.
Good Laboratory Practice: Regulations in KI, Design and protocols, logging.
Good tissue and good cell culture practice: Regulations and examples.
Good manufacturing practice: Legal aspects, designs and protocols, Logging, quality controls and examples.
Good clinical practice: Statistical planning and analysis, regulations, preparation for a clinical trial introductory overview.
Compiling the GLP-GMP-GCP map.
▪ Medical and Developmental Genetics, Course No: 2347
▪ Cell and Gene Therapy, Course No: 2444
▪ Cell Transplantation, Course No: 2557
▪ Transplantation Immunology, Course No: 2142
▪ Cartilage and Bone Biology, Course No: 2575
▪ Progenitor/Stem Cells and Regenerative Medicine, a Laboratory Course, Course No: 2364
▪ Mesenchymal Stem Cells -Biology and Clinical Potential, Course No: 2652
▪ Hematopoiesis and Hematopoietic Stem Cells -Health and Disease, Course No: 2717
▪ Central Concepts in Developmental Biology and Regenerative Medicine, Course No: 2699
▪ Practical Course in Microarray Data analysis, Course No:
▪ Regulatory RNAs in Development, Disease and Evolution, Course No: 2698
▪ Clinical Achievements of Reproductive Biology, Course No: 2291
▪ Cell biology of Diabetes and Obesity, Course No: new
▪ Epigenetics in Development, Course No: new
▪ Cell Signalling in Craniofacial Development, Course No: 2257
▪ Exploring Human Movement With Computerised Movement Analysis Systems, Course No: 2194
▪ Tissue and Motion – Clinical Evaluation of Function and Treatment, Course No: 1507
▪ Surgical Metabolism, Course No: coming
▪ Pharmacokinetics and Pharmacodynamics, Course No: coming
▪ Regenerative Medicine: Principles to practice, Course No: coming
▪ Human Pre-implantation Development and Pluripotent Stem Cells, Course No: coming
▪ Research Seminar Series and yearly Scientific Research Retreats (see archive and latest news).
2) Medical and developmental genetics
Course No: 2347, 1.5 hp
Course leader: Matti Nikkola
After the course, the student should be able to describe the basics of genetics and relate genetics to human health and disease.
This is an introductory course that will cover molecular, chromosomal, biochemical, developmental, cancer and medical genetics. Clinical case discussions will be included.
3) Cell and gene therapy
Course No: 2444, 1,5 hp
Course leader: Evren Alici
After the course, the student should be able to:
Explain mechanisms that are involved in viral gene integration in mammalian cells.
Apply their knowledge in molecular biology to genetic modification of mammalian cells. This means they should be able to develop cloning strategies for your gene of interest into a viral vector and propose a strategy for in vivo genetic manipulation.
Apply their knowledge in single gene disorders and develop treatment schemes for patients with these disorders using gene therapy.
The first part of the course will cover the basic aspects of gene regulation, cell culture, and gene transfer methods. In addition, the recent advances in vector development will be discussed. The second part of the course will deal with human studies, with special emphasis on the hematology field.
4) Transplantation Immunology
Course N:o 2575, 1.5 hp
Course leaders: Petter Höglund and Dan Hauzenberger. In collaboration with AII doctoral program.
After the course, each student is expected to be able to:
- Discuss immunological principles of relevance to transplantation and relate those to normal immunological reactions.
- Relate relevant methods used for investigations and diagnosis in relation to stem cell and organ transplantation.
- Discuss the cutting-edge of preclinical transplantation research.
- Discuss diagnostic principles before and after transplantation that are under development.
- Give a historical perspective of clinical and preclinical transplantation.
- Reflect over the future of clinical transplantation immunology.
The aim of the course is to give preclinical and clinical doctoral students within cell biology, immunology, transfusion medicine, hematology, transplantation surgery and internal medicine an updated and in-depth view of transplantation immunology. For this purpose, the biology, diagnostics and therapy of diseases in which transplantation is a treatment modality will be highlighted. An interdisciplinary view on research, diagnosis and therapy will be taken by integrating knowledged obtaind from different angles, such as cell biology, molecular biology, immunology, transfusion medicine, hematology, internal medicine, transplantation surgery and oncology. The course will thus connect preclinical and clinical transplantation research and focus on cell therapy, treatment of graft-versus-host disease and immunological monitoring after transplantation.
5) Cartilage and Bone Biology
Course No: 2142, 1,5 hp
Course leaders: Ola Nilsson and Göran Anderson
By providing up-to-date knowledge of cartilage and bone biology, the course aims to widen the perspective and the ability of the students to address basal and clinical questions in related fields of research.
The content of the course is divided into three parts:
This part provides an introductory overview on cartilage and bone development and structure, as well as cellular and matrix composition and function. The function and homeostasis during adulthood and aging will also be discussed.
In part II of the course, we will go into more depth of the different cell types, their unique biology, functions and differentiation. Methodolgies for culture, differentiation, mineralization etc will be demonstrated in order to provide basic tools as well as provide understanding of the unique characteristics of the different cell types.
Finally, selected examples of clinical applications that has stemmed from the basal knowledge of cartilage and bone biology will be presented.
1) Cell transplantation
Course No: 2557, 2 hp
Course leaders: Cecilia Götherström or Rachael Sugars
After the course, the student will be able to describe the basics of stem cell biology, different types of stem cells and the immunological mechanisms behind engraftment and rejection, and how tolerance develops after transplantation. The student will be able to extract and integrate information from state-of-the-art lectures in combination with overview articles and literature searches on the internet within the research field.
The course covers key concepts in translational cell transplantation research from the laboratory to the patient in the clinic. This includes a general overview of stem cell biology, transplantation immunology. Furthermore, an advanced summary of the latest events in the transplantation field will be given by experts. Ethical dilemmas in the transplantation area will be presented and discussed. The students take an active part, contribute and value discussions on ethical predicaments in the transplantation area.
2) Progenitor/Stem Cells and Regenerative Medicine: a Laboratory Course
Course no: 2364, 1.5 hp
Course leader: Cecilia Götherström
The aim of this course is for the student to learn the basic methodology of cultures of different cell types that are used in cell transplantation as well as the use of matrices and biomaterials. The student will also study laws and regulations, as well as GMP and GLP.
The course includes lectures and practical laboratory work. Areas that will be addressed are: GMP, GLP, EU tissue directive and Swedish tissue law. Principles of somatic and stem cell cultures. Culture and differentiation of human embryonic stem cells -principles and laboratory practice. Isolation and culture of human mesenchymal stem cells, including laboratory practice. Culture of human skin cell lines. Culture of adult and fetal hepatic cells.Handling and culture of hematopoietic stem cells. Handling of pancreatic islets.
3) Regenerative Medicine: Principles to Practice
Course no: new 1.5 hp
Course leaders at KI: Karl-Henrik Grinnemo and Cecilia Götherström
This is a collaborative course under development with Andre Terzic at the Mayo Clinic, Minnesota, US. Some of the lectures will be given by KI and Mayo Clinic via a video link. The idea is for the two Universities to complement each other in areas within regenerative medicine.
4) Stem Cells Engineering and Regenerative Medicine
Course No: 2603, 1.5 hp
Course leader: Ana Teixeira
After the completed course, the students can describe a number of novel technologies and approaches in stem cell research and regenerative medicine. Students can demonstrate an interdisciplinary understanding of central concepts in tissue engineering, biomaterials and stem cell science, and critically evaluate different methods and techniques used.
The course covers key aspects in stem cell research, ranging from fundamental mechanisms of cell function and developmental processes to controlling cell behavior for tissue engineering. Emphasis will be on the use of biomaterials and engineering approaches to control the cellular microenvironment and steer stem cell differentiation, both for fundamental understanding and in clinical translational applications. Networking with students and speakers is highly encouraged in this course, i.e. a dinner with the speakers and students will be held. A 2-day scientific symposium is s part of this course.
5) Clinical Achievements of Reproductive Biology
Course No: 2291, 1.5 hp
Course leader: Kenny Rodriguez-Wallberg
At the end of this course, participants should be able to have a comprehensive view of:
1. the key factors involved in fertilization and early development
2. their clinical impact in subfertility and infertility
3. the achievements of modern reproductive medicine
Biology of the gametes, fertilization and early embryo development, implantation, early pregnancy loss, ectopic pregnancy, repeated miscarriage. The female and male factor in infertility. Genetics in infertility, assisted reproduction techniques, the IVF laboratory. Fertility preservation for cancer patients, cryopreservation of gametes and gonadal tissue, ovarian transplantation, transplantation of the uterus.
6) Human pre- implantation development and pluripotent stem cells
Course No: new, 2 hp
Course leader: Outi Hovatta
At the conclusion of this course students should be have a good understanding of:
In vitro vs in vivo embryo development, culture system (culture media, plastics, gases, incubators, workstations, laboratory environment, input materials) and physical-chemical properties, the influence of the laboratory and clinic environment on embryo culture.
The morphology of oocytes, zygotes and cleavage stage embryos as well as morulae and blastocysts, and developmental milestones.
Predictors and biochemical tests of developmental potential. The use of ultrasound to assess oocyte quality.
Scoring of blastocysts for isolation of ICM. Derivation methods and culture systems and methods of embryonic stem cells. Methods to produce induced pluripotent stem cells.
Characterization of pluripotent stem cells and the importance of the pluripotency of these cells. Differentiations assay on stem cells and what is ongoing in this field.
Immune rejection and transplantation problems of pluripotent stem cells. Production of embryonic stem cells by somatic cell nuclei transfer or therapeutic cloning (SCNT).
Lectures: Sperm theory, embryology, genetics, genetic aspects of prenatal diagnostics and PGD, cloning techniques, methods for pluripotent stem cells (embryonic stem cells, induced pluripotent stem cells and SCNT).
Laboratory work: How to work with sperm, oocytes and embryos, manipulation and training with mouse oocytes and mouse embryo handling, micromanipulation techniques, presentation, micromanipulation training, ICSI and embryo biopsy. Culture of pluripotent stem cells.
7) Exploring Human Movement With Computerised Movement Analysis Systems
Course No: 2194, 3 hp
Course leader: Wim Grooten
At the end of the course, the student should:
be able to formulate scientific movement problems
be able to design adequate experiments for the above
be able to perform a movement scientific experiment
be able to analyse kinematic, kinetic and EMG data
be able to evaluate their results against other studies
be able to clearly present their results orally and in writing
After theoretical sessions, the students will work practically, analysing electormyographic, kinematic and kinetic data from a self-designed human movement experiment and present them written and orally.
8) Tissue and Motion – Clinical Evaluation of Function and Treatment
Course No: 1507, 1,5 hp
Course leaders: Lars Weidenhielm and Annika Kling Österlund
Kunna framställa och värdera en forskningsplan för ett projekt inom rörelseorganens sjukdomar. Ha lärt sig ett vetenskapligt och kritiskt förhållningssätt till den vetenskapliga litteraturen inom området för rörelseorganens sjukdomar och skador.
Klinisk utvärdering av funktion och behandling inom rörelseorganens sjukdomar och skador. Kunskaper om metoder, försöksplanering och statistisk bearbetning av material inom kliniska forskningsprojekt som berör rörelseorganens sjukdomar och skador.
9) Surgical Metabolism
Course No: new, 1.5 hp
Course leaders: Inga Tjäder and Anna Januszkiewicz
Metabolism plays an important role in the course of disease and affects both outcome and recovery. The objective of the course is that the participants gain a solid basic knowledge of metabolism, nutrition, how these are affected by disease, how they affect outcome and how these can be measured.
The course will teach basics in metabolism and nutrition, with a focus on surgical metabolism and clinical nutrition. In addition techniques, methods and study design to assess metabolism and the effects of nutrition will be discussed. The course will include lectures, group discussions and practical moments.
10) Pharmacokinetics and Pharmacodynamics
Course No: new, 1.5 hp
Course leaders: Jan Persson and Åke Norberg
The student will learn how to reason qualitatively about, and quantitatively calculate pk/pd processes based on physiology and pk/pd models. They will be able to discuss the relationship between pk/pd models and physiology.
Basic mathematical tools and mathematical models to describe and predict the time-course of drug concentrations in the body (pharmacokinetics) as well as the intensity of drug effects on the organism (pharmacodynamics). Iterative computer methods for nonlinear regression analysis, lecture-based teaching and hands-on workshop with computers.
11) Cell Signalling in Craniofacial Development
Course No: 2257, 1.5hp
Course leaders: Rachael Sugars and Mikael Wendel
The aim of the course is to give the students an overview of the current status of craniofacial biology, from cellular signalling, developmental and tissue regenerative perspectives.
After completion of the course the student should be able to:
- appreciate the complex nature of the signalling pathways involved in craniofacial development to ensure correct morphology,
- recall and provide examples of cellular signalling pathways involved in craniofacial development,
- compare and contrast the different signalling pathways involved in the development of various craniofacial structures,
- summarise the importance of stem cells in facilitating the establishment of regenerative therapies for craniofacial structures,
- evaluate the different methods of analysis used to study craniofacial tissues, reflect on how alterations and changes to these signalling pathways can affect the developmental processes and lead to defects/diseases in the craniofacial region.
Specifically, the course will examine the cellular signalling processes leading to the development of craniofacial tissues; from the skeletal elements to tooth morphogenesis, neural innervation to soft tissue formation. The course will also explore the potential to regenerate these tissues in the cases of disease or trauma.
The course will consist of lectures and seminars, along with methodology based workshops. Lectures and seminars from experts in the fields will cover: General craniofacial embryology and introduction to cellular signalling, Neural crest cell fate determination and cell signalling in embryonic development, Cellular signalling in skeletogenesis of craniofacial tissues, Tooth morphogenesis and hair follicle development, Pathways of cellular signalling, including BMP, FGF and Hedgehog in development, Neural innervation of craniofacial tissues and structures, Soft tissue formation, Biomaterials and stem cells for the regeneration of craniofacial tissues, Diseases affecting craniofacial development, Methodology workshops:, Methods of analysis – whole mount in situ, cell fate mapping, cell and organ culture methods, Clinical approaches to treating of craniofacial diseases and trauma.
12) Scientific Research Retreats and Research Seminar Series
Organisers: Doctoral program in Regenerative Medicine, Developmental Biology for Regenerative Medicine and the research Network Tissue and Motion
These activities will introduce the student to a mixture of regenerative medicine research, which will broaden the student’s knowledge of what is happening in the research field, and give new ideas for their own research. The student will also develop a professional and scientific critical thinking.